upec strain cft073  (ATCC)

Journal: PLOS Pathogens

Article Title: Single-cell analysis reveals an important role of CD137L + macrophages in the host response to uropathogenic Escherichia coli infection in the bladder

doi: 10.1371/journal.ppat.1013543

Figure Lengend Snippet: (A) Graphical illustration of the experimental setup. Bladders were harvested from both UPEC and control groups (in duplicate), then enzymatically digested to generate single-cell suspensions. Live CD45 + cells were FACS-sorted and loaded cells for scRNA-seq. This figure was created using Figdraw and is licensed with authorization from Figdraw ( www.figdraw.com ). (B) Nonlinear dimensionality reduction Uniform Manifold Approximation (UMAP) visualization of 24415 bladder CD45 + immune cells identified 13 different cell types after unsupervised clustering in healthy and UPEC infection group, respectively. Each point depicts a single cell, colored according to cell type designation. (C) Dot plots of gene expression level identified within CD45 + immune cell populations. (D) Summary of proportion of assigned cell types in healthy and UPEC infection group, respectively. (E) UMAP visualization of 16 distinct bladder monocyte clusters, myeloid populations colored in accordance of group. (F) Dot plots of gene expression level identified within myeloid populations. (G) Bar graph of relative abundance of each cluster of myeloid types healthy and UPEC infection conditions. (H) Differences in selected hallmark pathway activities scored with GSVA software. (I) Dot plots displaying the representative differentially enriched GOBP terms between Ms_2 ( Ccl2 hi Ms) and Ms_5 ( Tnfsf9 + Ms). (J) GSEA reveals bacterial infection associated signal pathways enriched in Ms_5 ( Tnfsf9 + Ms) compared with Ms_2 ( Ccl2 hi Ms).

Article Snippet: Uropathogenic Escherichia coli (UPEC) strain CFT073 was purchased from American type culture collection (700928).

Techniques: Control, Infection, Gene Expression, Software

Journal: PLOS Pathogens

Article Title: Single-cell analysis reveals an important role of CD137L + macrophages in the host response to uropathogenic Escherichia coli infection in the bladder

doi: 10.1371/journal.ppat.1013543

Figure Lengend Snippet: (A) CD137L + Ms in bladders from 8-week-old mice in UPEC and Control groups were analyzed by flow cytometry. Dot plots depict the gating strategy for CD137L + Ms. Graph shows the proportion of bladder CD137L + Ms ( n = 5). (B) CD137L + Ms in bladders from 8-week-old infected Tnfsf9 F/F Lyz2 +/+ and Tnfsf9 F/F Lyz2 Cre/+ mice were analyzed by flow cytometry. Dot plots depict the gating strategy for CD137L + Ms. Graph shows the proportion of bladder CD137L + Ms (n = 5). (C) Bacterial load was assessed 24 hours after infection ( n = 10). (D) The mRNA expression of Il1b , Il6 and Tnf in the bladder tissues were measured by RT-PCR ( n = 6). (E) H&E Staining of bladders from 8-week-old female Tnfsf9 F/F Lyz2 +/+ and Tnfsf9 F/F Lyz2 Cre/+ mice. Histology scores were assessed after infection ( n = 5). (F) Representative images of uroplakin3a in superficial bladder urothelium of Tnfsf9 F/F Lyz2 +/+ and Tnfsf9 F/F Lyz2 Cre/+ UTIs mice models ( n = 4). (G) The expression of IL-1β and TNF-α on macrophages between infected Tnfsf9 F/F Lyz2 +/+ and Tnfsf9 F/F Lyz2 Cre/+ mice were analyzed by flow cytometry. The graph depicts the expression of IL-1β and TNF-α on macrophages (n = 6).

Article Snippet: Uropathogenic Escherichia coli (UPEC) strain CFT073 was purchased from American type culture collection (700928).

Techniques: Control, Flow Cytometry, Infection, Expressing, Reverse Transcription Polymerase Chain Reaction, Staining

Journal: PLOS Pathogens

Article Title: Single-cell analysis reveals an important role of CD137L + macrophages in the host response to uropathogenic Escherichia coli infection in the bladder

doi: 10.1371/journal.ppat.1013543

Figure Lengend Snippet: (A) UMAP visualization of 16 distinct bladder lymphoid cells in UPEC and Control groups, colored according to cluster designation. (B) Dot plots of gene expression level identified within lymphoid cells. (C) Summary of proportion of assigned lymphoid cells in UPEC and Control groups, respectively. (D) Representative genes related to typical functions of Tregs in UPEC and Control groups. (E) Dot plots displaying the representative differentially enriched GOBP terms between Tregs taken before and after the infection. (F) GSEA reveals infection-related pathways enriched in Tregs after UPEC infection. Each dot represents one mouse; lines are medians. (G) Tregs in bladders from 8-week-old mice in UPEC and Control groups were analyzed by flow cytometry. Graph shows the proportion of bladder Tregs ( n = 5). (H) Tregs in bladder of Foxp3 DTR with DT injection or not were analyzed by flow cytometry. Graph shows the proportion of bladder Tregs. Dot plots depict the gating strategy for Tregs ( n = 4). (I) Bacterial load was assessed 24 hours after infection ( n = 10). (J) The mRNA expression of Il1b , Il6 and Tnf in the bladder tissues were measured by RT-PCR ( n = 4). (K) H&E Staining of bladders from Foxp3- DTR and normal mice after UPEC infection. Histology scores were assessed after infection ( n = 5). (L) Representative images of uroplakin3a in superficial bladder urothelium of Foxp3 DTR and normal UTIs mice models ( n = 4).

Article Snippet: Uropathogenic Escherichia coli (UPEC) strain CFT073 was purchased from American type culture collection (700928).

Techniques: Control, Gene Expression, Infection, Flow Cytometry, Injection, Expressing, Reverse Transcription Polymerase Chain Reaction, Staining

Journal: PLOS Pathogens

Article Title: Single-cell analysis reveals an important role of CD137L + macrophages in the host response to uropathogenic Escherichia coli infection in the bladder

doi: 10.1371/journal.ppat.1013543

Figure Lengend Snippet: (A) Selected significant signaling pathways were ranked based on their differences in overall information flow within the inferred networks between UPEC and Control groups. The top signaling pathways colored red are more enriched in UPEC group, the middle one colored black is equally enriched in UPEC and Control groups, and the bottom ones colored green are more enriched in Control group. (B) Violin plot showing the expression distribution of signaling genes involved in the inferred CD137 signaling network in UPEC and Control groups. (C) Heatmap shows the relative importance of each cell group based on the computed four network centrality measures of CD137 signaling network. (D-E) The expression of CTLA-4 and PD-1 on Tregs between infected Tnfsf9 F/F Lyz2 +/+ and Tnfsf9 F/F Lyz2 Cre/+ mice were analyzed by flow cytometry. The graph depicts the expression of CTLA-4 and PD-1 on Tregs (n = 5). (F-G) The expression of CTLA-4 and PD-1 on Tregs between infected Tnfsf9 F/F Itagx +/+ and Tnfsf9 F/F Itagx Cre/+ mice were analyzed by flow cytometry. The graph depicts the expression of CTLA-4 and PD-1 on Tregs (n = 5).

Article Snippet: Uropathogenic Escherichia coli (UPEC) strain CFT073 was purchased from American type culture collection (700928).

Techniques: Protein-Protein interactions, Control, Expressing, Infection, Flow Cytometry

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Antifungal, anti-biofilm, and anti-hyphal properties of N -substituted phthalimide derivatives against Candida species

doi: 10.3389/fcimb.2024.1414618

Figure Lengend Snippet: Impact of N -butylphthalimide (NBP) on the biofilm formation and planktonic cell growth of Candida species. By cultivating for 24 h at 37°C under static conditions in 96-well polystyrene plates, the planktonic cell growth of Candida albicans DAY185 (A) and the anti-biofilm activities of NBP against C. albicans 10231 (B) , Candida parapsilosis (C) , Staphylococcus epidermidis (D) , Vibrio parahaemolyticus (E) , uropathogenic Escherichia coli (F) , Staphylococcus aureus ATCC 6538 (G) , and polymicrobial biofilms (H) were investigated. *p<0.05 vs untreated controls (None).

Article Snippet: The uropathogenic E. coli (UPEC) O6:H1 strain CFT073 (ATCC 700928) was streaked on Luria–Bertani (LB) agar and incubated in nutrient broth at 37°C for 12 h for overnight culture.

Techniques: